Conversion of AFLP Markers Surrounding a QTL, rhg-t1, for Soybean Cyst Nematode Resistance into PCR-based Markers

Engineered Soybean Cyst Nematode Resistance

QTLs Associated with Resistance to Soybean Cyst Nematode in Soybean: Meta-Analysis of QTL Locations

Soybean cyst nematode (SCN) (Heterodera glycines Ichinohe) is the most important pest of soybean [Glycine max (L.) Merr.] in the world. A total of 17 quantitative trait locus (QTL) mapping papers and 62 marker–QTL associations have been reported for resistance to soybean cyst nematode in soybean. Conflicting results often occurred. The objectives of this study were to: (i) evaluate evidence for...

A Decade of QTL Mapping for Cyst Nematode Resistance in Soybean

example, Rao-Arelli et al. (1992b) showed that several isolates of H. glycines were initially classified as one Soybean cyst nematode (SCN) (Heterodera glycines Ichinohe), race by this scheme. However, when later tested with the most destructive pest of soybean [Glycine max (L.) Merrill], is estimated to be responsible for almost nine million megagrams in resistant genotypes other than the stan...

A new and versatile method for the successful conversion of AFLP markers into simple single locus markers.

Genetic markers can efficiently be obtained by using amplified fragment length polymorphism (AFLP) fingerprinting because no prior information on DNA sequence is required. However, the conversion of AFLP markers from complex fingerprints into simple single locus assays is perceived as problematic because DNA sequence information is required for the design of new locus-specific PCR primers. In a...

Conversion of barley SNPs into PCR-based markers using dCAPS method

Molecular genetic research relies heavily on the ability to detect polymorphisms in DNA. Single nucleotide polymorphisms (SNPs) are the most frequent form of DNA variation in the genome. In combination with a PCR assay, the corresponding SNP can be analyzed as a derived cleaved amplified polymorphic sequence (dCAPS) marker. The dCAPS method exploits the well-known specificity of a restriction e...